| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 9902321 | Journal of Immunological Methods | 2005 | 13 Pages |
Abstract
Stem cell factor (SCF) stimulated cell proliferation, but not as effectively as rHuEPO. Antibodies to SCF were not able to inhibit the proliferative response induced by EPO and vice versa, confirming the specificity of the assay for antibodies to EPO. High EPO levels can impact both the radioimmunoprecipitation and neutralization assays to produce a false negative result. However, the impact can be mitigated by the large dilutions used in the neutralization assay.
Keywords
GM-CSFrGM-CSFBQLradioimmunoprecipitationrHuEPOSCFNHSCRFEPOBioassayerythropoietinRecombinant human erythropoietinEnzyme-linked immunosorbent assayELISAThymidine uptakepercent coefficient of variationnormal human serumCytokinesIdentificationgranulocyte–macrophage colony-stimulating factorStem Cell FactorChronic renal failureAntibodyRIPAnemiaquality control
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Biotechnology
Authors
Marian Kelley, Cianna Cooper, Aileen Matticoli, Anthony Greway,