Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9902340 | Journal of Immunological Methods | 2005 | 11 Pages |
Abstract
We conclude that (i) the use of HLA-transfected K562 cells allows to dissect antigen-specific T cell responses to partial responses associated with defined HLA class I alleles and (ii) transfection of in vitro transcribed RNA fragments allows to identify immunogenic regions of a given antigen. The latter technique bypasses the need of prior cloning and sequencing of cDNA fragments, reduces the number of synthetic peptides to be tested and thus saves both costs and time.
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Authors
Cedrik M. Britten, Ralf G. Meyer, Claudine Graf, Christoph Huber, Thomas Wölfel,