Efficient elutriation of monocytes within a closed system (Elutra™) for clinical-scale generation of dendritic cells

The Elutra™ separator allows for fast and easy enrichment of monocytes within a closed system. Subsequently, elutriated monocytes can be successfully cultured into phenotypically and functionally mature DC for immunotherapeutic approaches. The method neither requires a density gradient step to enrich PBMC from leucapheresis products nor does it apply (xenogeneic) antibodies to target monocytes. Isolation of monocytes with Elutra™ may greatly facilitate future DC-based vaccination approaches.