Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9912063 | Cellular Immunology | 2005 | 9 Pages |
Abstract
The majority of T lymphocytes that infiltrate psoriatic lesions express cutaneous lymphocyte antigen (CLA), a skin homing receptor involved in the influx of memory T cells to cutaneous sites. We investigated CLA expression on normal human peripheral blood mononuclear cells (PBMCs) and evaluated its association with IL-12 receptors, chemokine receptor, CXCR3, and IL-2Rα. PBMCs were stimulated in vitro with or without polyclonal activators (mitogen, or superantigens, or anti-CD3 + anti-CD28) in the presence or absence of exogenous rhIL-12. The percentage of CLA+ T lymphocytes increased significantly after superantigen stimulation compared to anti-CD3 + anti-CD28 or mitogen activation. The majority of activation induced CLA+ T lymphocytes co-expressed IL-12Rβ1, IL-12Rβ2, CXCR3, and CD25 in the presence of rhIL-12. Our results indicate that CLA expression on activated T lymphocytes is IL-12 and activation dependent and correlates with the expression of IL-12 receptors, IL-2Rα, and CXCR3. Monitoring the levels of Th1 differentiation markers such as CXCR3 and IL-12Rβ2 along with activation marker, CD25 on skin homing CLA+ T lymphocytes may provide insight into the mechanism of action of immunotherapies directed against Th1 type skin inflammatory diseases.
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Cell Biology
Authors
Manjula Reddy, Cuc Davis, Jackson Wong, Uma Prabhakar,