Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9914951 | Molecular and Cellular Endocrinology | 2005 | 11 Pages |
Abstract
Over a 10-day culture period both structural integrity and glucose-stimulated insulin release (GSIR) were preserved in islets maintained within the RCCS whilst those held under CSC conditions exhibited progressive fragmentation and rapid loss of secretory function. In addition, intentionally dissociated islet cells maintained within the RCCS demonstrated the ability to re-aggregate and form tight islet-like structures with enhanced secretory capacity compared to whole islets maintained in static culture. These findings suggest a novel use for the RCCS and illustrate its potential as an experimental tool for in vitro study of human islet/β-cell physiology.
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Authors
Hilary E. Murray, Michelle B. Paget, Richard Downing,