Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
9915054 | Molecular and Cellular Endocrinology | 2005 | 8 Pages |
Abstract
Relative gene expression of the estrogen receptors (ER)-α (NR3A1) and ER-β (NR3A2) along with progesterone receptors PR-A and PR-B (NR3C3) was determined by quantitative real-time PCR in a previously characterized panel of paired human prostate tumor and surrounding unaffected tissue (Prostate 54:275). In approximately half of these cases, a 10-fold or greater reduction in the relative mRNA levels of ER-β but not ER-α was found in the cancer as compared to normal tissue, which was also observed with unpaired samples. Immunohistochemical staining for ER-β and ER-α closely paralleled mRNA expression patterns for both receptors in paired samples. Reduced relative expressions of PR-B and total PR-A and PR-B isoforms were also observed in prostate tumor as compared to unaffected tissue, implying a potential role of PR in prostate tissue. The relative decrease in ER-β is greater than that observed in prior studies, suggesting that paired samples more accurately reflect differences within individual cases. These findings favor the concept that ER-β mediates anti-proliferative signals and its loss in prostatic tumor promotes proliferation of these cells.
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Authors
Qing Ji, Paul I. Liu, Yahya Elshimali, Andrew Stolz,