Superficial and deep layer muscle fibre properties of the mouse masseter before and after weaning

To clarify changes in the properties of the masseter muscle superficial and deep layer muscle fibres, which initiate masticatory movement, myosin heavy chain isoforms were evaluated based on immunohistochemistry at the transcription level in male mice both before and after weaning. In the results, MHC-2b isoforms, the isoforms with the fastest contraction speed, were observed in the superficial layer after weaning. However, MHC-2a isoforms with slower contraction speeds were not apparent. By contrast, in the deep layer, MHC-2a isoforms were present, as were MHC-2b isoforms, however, there were fewer MHC-2b isoforms present than in the superficial layer. The most rapid movement in the mouse mandible was observed anteroposteriorly during mastication. As the superficial layer of the masseter muscle runs parallel to the direction of mandibular movement, the presence of MHC-2b isoforms in it is consistent. The presence of MHC-2a isoforms in the deep layer, lying at right angles to the direction of mastication movement, is consistent with the positional adjustment of the mandible contributed by the deep layer muscle fibres during masticatory movement. We therefore conclude that complicated masticatory movement is achieved by the presence of various muscle bundles within the masseter, each carrying out different roles.