Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10129695 | Microbial Pathogenesis | 2018 | 41 Pages |
Abstract
Brucellosis is an infectious disease that brings enormous economic burdens for developing countries. The Brucella melitensis (B. melitensis) M5-90 vaccine strain (M5-90) has been used on a large scale in China, but may cause abortions if given to pregnant goats or sheep subcutaneously during the late stages of gestation. Moreover, the vaccine M5-90 cannot differentiate natural from vaccinated infection. Therefore, a safer and more potent M5-90 vaccine is required. In this study, a vjbR mutant of M5-90 (M5-90ÎvjbR) was constructed and overcame these drawbacks. M5-90ÎvjbR strain showed reduced survival capability in murine macrophages (RAW 264.7) and BALB/c mice and induced high protective immunity in mice. In addition, M5-90ÎvjbR induced an anti-Brucella-specific immunoglobulin G (IgG) response and stimulated the expression of gamma interferon (INF-γ) and interleukin-4 (IL-4) in vaccinated mice. Furthermore, M5-90ÎvjbR induced IgG response and stimulated the secretion of IFN-γ and IL-4 in immunized sheep. Moreover, the VjbR antigen allowed serological differentiation between infected and vaccinated animals. These results suggest that M5-90ÎvjbR is an ideal live attenuated and efficacious live vaccine candidate against B. melitensis 16â¯M infection.
Keywords
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Microbiology
Authors
Zhiqiang Li, Shuli Wang, Hui Zhang, Li Xi, Jinliang Zhang, Xiaogen Zhang, Qingfeng Zhou, Jihai Yi, Min Li, Weihua Zhang, Junbo Zhang,