Green and eco-friendly route for the synthesis of Ag@Vitamin B9-LDH hybrid and its chitosan nanocomposites: Characterization and antibacterial activity

Firstly, Ag nanoparticle (Ag-NP) was synthesised with environmentally-friendly and green media extracted from Rosmarinus officinalis plants. The structure of Ag-NP was confirmed with the absorption peak related to the Surface Plasmon Resonance at 432 nm through UV-Vis analysis. Subsequence, Ag-NP was composited with pre-synthesised vitamin B9 (VB9) intercalated Layered Double Hydroxide (VB9-LDH) and Ag@VB9-LDH with different percentages was incorporated in the chitosan (CS) as bio-polymeric matrix. The improvement in the physio-chemical properties of prepared nanocomposites (NCs) was confirmed with different spectroscopic techniques. Finally, the effect of polymeric matrix on the antibacterial activity of this compound was examined against two Gram-negative and Gram-positive bacteria. The results of antibacterial test showed the remarkable antibacterial activity for all samples against both bacteria and NC 6 wt% showed greater inhibition zone diameter (19 mm and 11 mm) in compared to the pristine Ag@VB9-LDH (11 mm and 8 mm) for Gram-negative and Gram-positive, respectively.