Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10156670 | Biochemical and Biophysical Research Communications | 2018 | 7 Pages |
Abstract
AMP-activated protein kinase (AMPK) functions as a cellular energy sensor by monitoring the cellular AMP:ATP ratio and plays a central role in cellular and whole-body energy homeostasis. Recent studies have suggested that AMPK also contribute to cell cycle regulation, but its role in this field remains almost elusive. In the present study, we report that AMPKα1 was transiently activated during G1/S transition phase in NIH3T3 cells in the absence of any metabolic stress. Inhibition of AMPK activity at G1/S transition phase completely blocked cells from entering S phase; in contrast, persistent activation of AMPK at G1/S transition phase allowed cells to normally enter S phase, but these cells failed to proceed to G2/M phase, stacking at S phase. We further demonstrated that activation of AMPK at G1/S transition phase depends on Ca2+ transients and CaMKKβ activity, but not on energy status. Collectively, these data indicate that temporal regulation of AMPK is required for proper control of S phase in NIH3T3 cells.
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Authors
In-Ja Park, Quynh Hoa Tran, Ain Syafiza Mohd Amin, Thanh Lan Chu, Goowon Yang, Wonchae Choe, Insug Kang, Sung Soo Kim, Joohun Ha,