Modulation of Kv3.1b potassium channel level and intracellular potassium concentration in 158N murine oligodendrocytes and BV-2 murine microglial cells treated with 7-ketocholesterol, 24S-hydroxycholesterol or tetracosanoic acid (C24:0)

Our data establish that 7KC, 24S-OHC and C24:0 increase intracellular K+ concentration ([K+]i) in both 158N and BV-2 cells. The highest [K+]i were detected on 158N cells. Increased level of Kv3.1b was shown on 158N cells under treatment with 7KC and 24S-OHC but not with C24:0. This effect was less important on BV-2 cells. Positive correlations between Kv3.1b level and [K+]i were observed. In addition, the [K+]i and the level of Kv3.1b along with increasing percentage cells with depolarized mitochondria, ROS overproducing cells and dead cells.152