Hypoxia-stressed cardiomyocytes promote early cardiac differentiation of cardiac stem cells through HIF-1Î±/Jagged1/Notch1 signaling

Article ID	Journal	Published Year	Pages	File Type
10158173	Acta Pharmaceutica Sinica B	2018	24 Pages	PDF

Abstract

Hypoxia stress provokes Jagged1 expression in cardiomyocytes mainly through ERK signaling, subsequently leads to activation of Notch1 signaling in c-Kit+ cardiac stem cells (CSCs) via direct cell contact, which in turn favors cardiogenic lineage differentiation in CSCs.205

Keywords

NF-κB Notch1 signaling YC-1 MACS HRE CMS HIF-1α DAPI ERK vWF RT-PCR BMSCs CSCs GFP STAT3 PBS Jnk FBS FITC qPCR Janus kinase 4′,6-diamidino-2-phenylindole 5-bromo-2′-deoxyuridine c-Jun N-terminal kinase DMSO MOI Quantitative PCR Myocardial infarction BrdU Cell differentiation Notch intracellular domain Dimethyl sulfoxide reverse transcription PCR fetal bovine serum cardiac stem cell Cardiac stem cells Bone marrow stem cells hypoxia-inducible factor 1α Von Willebrand factor Hypoxia responsive element nuclear factor κB phycoerythrin phosphate buffer saline fluorescein isothiocyanate Cardiomyocytes signal transducer and activator of transcription 3 magnetic-activated cell sorting Hypoxia green fluorescent protein multiplicity of infection JAK extracellular signal-regulated kinase