| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10160575 | Biochemical Engineering Journal | 2011 | 7 Pages |
Abstract
ⶠSoluble and functional scFv-Fc is expressed in E. coli trxB/gor double mutant. ⶠSolubility of scFv-Fc in the cytoplasm is improved by co-expression of GroELS. ⶠProductivity of more than 10 mg/L is achieved in shake-flask culture.
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Authors
Hiroyuki Sonoda, Yoichi Kumada, Tomohisa Katsuda, Hideki Yamaji,