Improvement of medium composition for heterotrophic cultivation of green microalgae, Tetraselmis suecica, using response surface methodology

▶ Medium for heterotrophic growth of Tetraselmis suecica cells was optimized using CCD design. ▶ Medium: glucose, 5.8 g/L; peptone, 9 g/L; yeast extract, 4.5 g/L; meat extract, 3 g/L. ▶ T. suecica cells, yield and productivity: 28.8 g/L, 9.1 g cell/g glucose and 0.2 g/L/h. ▶ Cells in heterotrophic (28.8 g/L) thrice higher than photoautotrophic (8.4 g/L).