Prediction of protein partition in polymer/salt aqueous two-phase systems using the modified Wilson model

In the modelling methodology adopted here, special emphasis on the so-called “charge effects” to the protein partition was given. To our knowledge, no experimental information is available in the literature that allows to estimate the interaction parameters between these macromolecules and the components present in the aqueous two-phase systems (water, salts and polymer). Thus, the deviations observed between calculated and experimental protein partition are mainly due to some assumptions made in the predictive methodology.