Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10162200 | Journal of Pharmaceutical Sciences | 2015 | 7 Pages |
Abstract
Aluminum salts have a long history as safe and effective vaccine adjuvants. In addition, aluminum salts have high adsorptive capacities for vaccine antigens and adjuvant molecules, for example, Toll-like receptor 4 (TLR4) agonists. However, the physicochemical properties of aluminum salts make direct quantitation of adsorbed molecules challenging. Typical methods for quantifying adsorbed molecules require advanced instrumentation, extreme sample processing, often destroy the sample, or rely on an indirect measurement. A simple, direct, and quantitative method for analysis of adsorbed adjuvant molecules is needed. This report presents a method utilizing Fourier transform infrared spectroscopy with a ZnSe-attenuated total reflectance attachment to directly measure low levels (< 30 μg/mL) of TLR4 agonists adsorbed on aluminum salts with minimal sample preparation. © 2014 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 104:768-774, 2015
Keywords
Related Topics
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Pharmacology, Toxicology and Pharmaceutical Science
Drug Discovery
Authors
Quinton M. Dowling, Alicia M. Schwartz, Thomas S. Vedvick, Christopher B. Fox, Ryan M. Kramer,