Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10162560 | Journal of Pharmaceutical Sciences | 2014 | 9 Pages |
Abstract
The aggregation behavior and critical aggregation concentration (CAC) values of daptomycin in aqueous solutions were evaluated under the external factors of pH, temperature, daptomycin concentration, and calcium ions concentration by using the complementary characterization techniques, fluorescence, dynamic and static light scattering, and nuclear magnetic resonance (NMR) spectroscopy. On the basis of the intrinsic fluorescence resonance energy transfer of daptomycin, the CAC values were identified by an upward inflection of the fluorescence emission from Kyn-13 at 460Â nm. The pH-dependent CAC values were determined to be 0.14Â mM at pH 3.0, 0.12Â mM at pH 4.0, and 0.20Â mM at pH 2.5 and 5.0. The CAC values obtained by fluorescence spectroscopy were confirmed by dynamic light scattering and NMR spectroscopy.
Keywords
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Authors
Jiang Qiu, Lee E. Kirsch,