Wpływ ostrej niewydolności oddechowej na zmiany ilościowe limfocytów CD3+, CD4+, CD8+ i HLA-DR+ u noworodków urodzonych przedwcześnie z masÄ ciała powyżej 1500 g

The aim of this study was to evaluate in peripheral vein blood the percentage and number of CD3+ lymphocytes and their subpopulations CD4+, CD8+ and HLA-DR+ in premature neonates with birth weight over 1500 g. The study population comprised of 17 girls and 30 boys with gestational age from 29 to 37 weeks and birth weight from 1540 to 2750 g, that were divided into two groups according to respiratory failure: I - 28 without respiratory failure and II - 19 with respiratory failure. The immunological analysis was made using flow cytometer and monoclonal antibodies (Becton Dickinson). The mean percentage and number of CD3+ lymphocytes and CD4+ subpopulation was significantly higher in neonates without respiratory failure in comparison to mean values of neonates from II group. The quantitative changes of CD+ and CD4+ strictly associated with severity of clinical state of premature neonates may unfavorably influence their defense ability.