Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10228617 | Biomaterials | 2013 | 10 Pages |
Abstract
Mesenchymal stem cells (MSCs) have been used for cell therapy in various experimental disease models. However, the regenerative potential of MSCs from different tissue sources and the influence of the tissue niche have not been investigated. In this study, we compared the regenerative potential of dental pulp, bone marrow and adipose tissue-derived CD31â side population (SP) cells isolated from an individual porcine source. Pulp CD31â SP cells expressed the highest levels of angiogenic/neurotrophic factors and had the highest migration activity. Conditioned medium from pulp CD31â SP cells produced potent anti-apoptotic activity and neurite outgrowth, compared to those from bone marrow and adipose CD31â SP cells. Transplantation of pulp CD31â SP cells in a mouse hindlimb ischemia model produced higher blood flow and capillary density than transplantation of bone marrow and adipose CD31â SP cells. Motor function recovery and infarct size reduction were greater with pulp CD31â SP cells. Pulp CD31â SP cells induced maximal angiogenesis, neurogenesis and pulp regeneration in ectopic transplantation models compared to other tissue sources. These results demonstrate that pulp stem cells have higher angiogenic, neurogenic and regenerative potential and may therefore be superior to bone marrow and adipose stem cells for cell therapy.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
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Authors
Ryo Ishizaka, Yuki Hayashi, Koichiro Iohara, Masahiko Sugiyama, Masashi Murakami, Tsubasa Yamamoto, Osamu Fukuta, Misako Nakashima,