Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10228890 | Biomaterials | 2013 | 8 Pages |
Abstract
We report a microfluidic array for investigating and quantitatively analyzing human neural stem cell (hNSC) self-renewal and differentiation in an in vivo-like microenvironment. NSC niche conditions, including three-dimensional (3D) extracellular matrices and low oxygen tension, were effectively reconstituted in the microfluidic array in a combinatorial manner. The array device was fabricated to be detachable, rendering it compatible with quantitative real-time polymerase chain reaction for quantifying the effects of the biomimetic conditions on hNSC self-renewal and differentiation. We show that throughput of 3D cell culture and quantitative analysis can be increased. We also show that 3D hypoxic microenvironments maintain hNSC self-renewal capacity and direct neuronal commitment during hNSC differentiation.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Kisuk Yang, Sewoon Han, Yoojin Shin, Eunkyung Ko, Jin Kim, Kook In Park, Seok Chung, Seung-Woo Cho,