Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10229048 | Biomaterials | 2013 | 10 Pages |
Abstract
Metastasis is one of the greatest challenges in cancer treatment. In this study, a bioreducible polymer, Tween 85-s-s-polyethyleneimine 2K (TSP), was synthesized and used as a non-viral gene vector for p65 shRNA to block NF-κB signaling pathway, thereby inhibiting the growth and metastasis of breast cancer. The TSP/p65 shRNA complex nanoparticles (TSNs) could significantly down-regulate p65 expression in breast cancer cells due to the rapid degradation of TSP with prompt shRNA release, and consequently not only inhibit cell proliferation and invasion, but also induce cell apoptosis and disrupt the tube formation. Most importantly, TSNs showed high accumulation in tumor and almost completely inhibited the growth and metastasis of the breast cancer xenograft in nude mice induced by MDA-MB-435 cells. All these results indicated the promising of TSP as a non-viral gene vector to knock down p65 expression and inhibit the growth and metastasis of breast cancer.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Jisheng Xiao, Xiaopin Duan, Qi Yin, Zehong Miao, Haijun Yu, Chunying Chen, Zhiwen Zhang, Jun Wang, Yaping Li,