Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10229229 | Biomaterials | 2012 | 10 Pages |
Abstract
Laminin-111, a multifunctional matrix protein, has diverse biological functions. Previously, we have identified various biologically active sequences in laminin-111 by a systematic peptide screening. We also demonstrated that peptide-conjugated chitosan matrices enhance the biological functions of the active sequences and are useful as a scaffold. Here, we conjugated sixty biologically active laminin-111 peptides onto chitosan matrices. The twenty-nine peptide-chitosan matrices promoted various biological activities, including cell attachment, spreading, and neurite outgrowth. The biological activities of peptide-chitosan matrices depend on the peptide. These peptide-chitosan matrices are categorized into six groups depending on their biological activities. Next, we conjugated five active peptides, which showed strong cell attachment activity in the each group, onto a single chitosan matrix to mimic the multiple activities of laminin-111. The mixed peptides-chitosan matrix significantly promoted cell attachment and cell spreading over that observed with the individual peptides. We also demonstrated that a mixed peptides-chitosan matrix, using four neurite outgrowth-promoting peptides each from a different group, enhanced the activity. These data suggest that the mixed peptides synergistically induce laminin-like biological activities on a chitosan matrix. The active peptides-chitosan matrices described here have potential for use as biomaterial for tissue engineering and regeneration.
Keywords
HDFMBSBMENGFDAPIEHSFMOCFAKFBSEngelbreth-Holm-SwarmDMFECM4,6-diamidino-2-phenylindole9-fluorenylmethoxycarbonylBSAECM (extracellular matrix)bovine serum albuminIntegrinESCScaffoldfetal bovine serumEmbryonic stem cellbasement membrane extractnerve growth factorhuman dermal fibroblastExtracellular matrixPeptideCell adhesionChitin/chitosanfocal adhesion kinase
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Kentaro Hozumi, Ayano Sasaki, Yuji Yamada, Dai Otagiri, Kazuki Kobayashi, Chikara Fujimori, Fumihiko Katagiri, Yamato Kikkawa, Motoyoshi Nomizu,