Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10229252 | Biomaterials | 2012 | 13 Pages |
Abstract
Cancer immuno-gene therapy is an introduction of nucleic acids encoding immunostimulatory proteins, such as cytokine interleukin 12 (IL-12), into somatic cells to stimulate an immune response against a tumor. Various methods can be used for the introduction of nucleic acids into cells; magnetofection involves binding of nucleic acids to magnetic nanoparticles with subsequent exposure to an external magnetic field. Here we show that surface modified superparamagnetic iron oxide nanoparticles (SPIONs) with a combination of polyacrylic acid (PAA) and polyethylenimine (PEI) (SPIONs-PAA-PEI) proved to be safe and effective for magnetofection of cells and tumors in mice. Magnetofection of cells with plasmid DNA encoding reporter gene using SPIONs-PAA-PEI was superior in transfection efficiency to commercially available SPIONs. Magnetofection of murine mammary adenocarcinoma with plasmid DNA encoding IL-12 using SPIONs-PAA-PEI resulted in significant antitumor effect and could be further refined for cancer immuno-gene therapy.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Sara Prijic, Lara Prosen, Maja Cemazar, Janez Scancar, Rok Romih, Jaka Lavrencak, Vladimir B. Bregar, Andrej Coer, Mojca Krzan, Andrej Znidarsic, Gregor Sersa,