| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10230102 | Biomaterials | 2005 | 6 Pages |
Abstract
Poly(N-isopropylacrylamide) has a thermoresponsive phase transition: at 32°C water-insoluble (hydrophobic) and water-soluble (hydrophilic) states interconvert. Adsorption of antibody onto PNIPAAm-g-PP membrane at 37°C and its desorption at 4°C was verified by fluorescence-microscopy of the PNIPAAm-g-PP membrane after soaking it in fluorescein-conjugated goat anti-mouse IgG in phosphate-buffered saline. PNIPAAm-g-PP membranes containing adsorbed anti-mouse CD80 monoclonal antibody preferentially captured mouse-CD80 transfected cells at 37°C compared with membranes lacking antibody or containing anti-mouse CD86 monoclonal antibody. Detachment of captured cells from PNIPAAm-g-PP membranes was facilitated by washing at 4°C because of the thermoresponsive phase transition of PNIPAAm. With this method, mouse CD80- or mouse CD86-transfected cells were enriched from a 1:1 cell suspension to 72% or 66%, simply and with high yield.
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Authors
Aiko Okamura, Midori Itayagoshi, Taeko Hagiwara, Manae Yamaguchi, Toshiyuki Kanamori, Toshio Shinbo, Pi-Chao Wang,