Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10230116 | Biomaterials | 2005 | 8 Pages |
Abstract
We developed a novel technique of hybridizing calcium phosphate (CaP) with bioorganic soft tissue using an alternating soaking process. By this technique, we hybridized CaP with a grafted tendon tissue to bond with a bone tunnel. Tendons were soaked in Ca and NaHPO4 solutions alternately for 10 min. Needle-like CaP crystals 30-50 nm in length including low-crystalline apatite were deposited on and between collagen fibrils from the surface to 200 μm deep in the tendon. In light and transmission electron microscopic images, osteoclast-like cells and osteoblasts appeared on the implanted tendon and osteoid was observed on the tendon surface at 1 week postoperatively. At 2 weeks postoperatively, osteoclast-like cells resolved the tendon by forming Howship's lacuna-like spaces on the surfaces and osteoblasts formed osteoid in these spaces. Direct bonding between the implanted tendon and the newly formed bone was observed. At 3 weeks postoperatively, thick newly formed bone firmly bonded to tendon surface. From these results, we conclude that the tendons prepared by an accelerated CaP hybridization method efficiently enhance osteoclast-like cells and osteoblasts to bond the implanted tendons to newly formed bone.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Hirotaka Mutsuzaki, Masataka Sakane, Atsuo Ito, Hiromi Nakajima, Shinya Hattori, Yutaka Miyanaga, Junzo Tanaka, Naoyuki Ochiai,