Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231324 | Biomolecular Engineering | 2005 | 4 Pages |
Abstract
The high affinity (kd = â¼10â15 M) of streptavidin and avidin for biotin is key to a large number of biological applications and is essentially irreversible unless the complex is exposed to harsh conditions (e.g. heat (100 °C for 10 min)), detergents, and/or denaturants which damage macromolecules. Thus, high binding affinity becomes a disadvantage when a biotinylated target must be released for further processing. This work describes relatively mild conditions that release biotin and mono- and bis-biotinylated macromolecules from immobilized streptavidin on monodispersed magnetic beads.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Giang H. Nguyen, Jaqueline S. Milea, Anurag Rai, Cassandra L. Smith,