Article ID Journal Published Year Pages File Type
10231324 Biomolecular Engineering 2005 4 Pages PDF
Abstract
The high affinity (kd = ∼10−15 M) of streptavidin and avidin for biotin is key to a large number of biological applications and is essentially irreversible unless the complex is exposed to harsh conditions (e.g. heat (100 °C for 10 min)), detergents, and/or denaturants which damage macromolecules. Thus, high binding affinity becomes a disadvantage when a biotinylated target must be released for further processing. This work describes relatively mild conditions that release biotin and mono- and bis-biotinylated macromolecules from immobilized streptavidin on monodispersed magnetic beads.
Related Topics
Physical Sciences and Engineering Chemical Engineering Bioengineering
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