Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231406 | Biotechnology Advances | 2015 | 10 Pages |
Abstract
Organic pollutants, including xenobiotics, are often persistent and toxic organic compounds resulting from human activities and released in large amounts into terrestrial, fluvial and marine environments. However, some microbial species which are naturally exposed to these compounds in their own habitat are capable of degrading a large range of pollutants, especially poly-aromatic, halogenated and polyester molecules. These microbes constitute a huge reservoir of enzymes for the diagnosis of pollution and for bioremediation. Most are found in highly complex ecosystems like soils, activated sludge, compost or polluted water, and more than 99% have never been cultured. Meta-omic approaches are thus well suited to retrieve biocatalysts from these environmental samples. In this review, we report the latest advances in functional metagenomics aimed at the discovery of enzymes capable of acting on different kinds of polluting molecules.
Keywords
PBSADGGEPESDBPPCBPHBABTSPAHPLAPCLdibutyl phthalateAMDSIPPBS2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)3,5,6-Trichloro-2-pyridinolGC–MSPollutantsPersistent organic pollutantDenaturing gradient gel electrophoresisPolychlorinated biphenylAcid mine drainagegas chromatography–mass spectrometryScreeningTCPMetagenomicshydrocarbonPolycyclic aromatic hydrocarbonspolymerase chain reactionPCRpopPETStable isotope probingPolyethylene terephthalatePolylactic acidPolyhydroxybutyratePolycaprolactonePolyurethanehigh performance liquid chromatographyHPLCEnzyme discovery
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Lisa Ufarté, Ãlisabeth Laville, Sophie Duquesne, Gabrielle Potocki-Veronese,