Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231538 | Biotechnology Advances | 2014 | 11 Pages |
Abstract
G-protein coupled receptors (GPCRs) are involved in regulating most of physiological actions and metabolism in the bodies, which have become most frequently addressed therapeutic targets for various disorders and diseases. Purified GPCR-based drug discoveries have become routine that approaches to structural study, novel biophysical and biochemical function analyses. However, several bottlenecks that GPCR-directed drugs need to conquer the problems including overexpression, solubilization, and purification as well as stabilization. The breakthroughs are to obtain efficient protein yield and stabilize their functional conformation which are both urgently requiring of effective protein synthesis system methods and optimal surfactants. Cell-free protein synthesis system is superior to the high yields and post-translation modifications, and early signs of self-assembly peptide detergents also emerged to superiority in purification of membrane proteins. We herein focus several predominant protein synthesis systems and surfactants involving the novel peptide detergents, and uncover the advantages of cell-free protein synthesis system with self-assembling peptide detergents in purification of functional GPCRs. This review is useful to further study in membrane proteins as well as the new drug exploration.
Keywords
SDCConALCPDcytBMbCDGPCRsORSSf21Sample displacement chromatographyT4LMBPPS-IDDME. coliG-protein coupled receptorsn-Dodecyl-β-D-maltosideExtractEscherichia coliCell-freeTAARCytochrome bphotosystem ILipid cubic phasetrigger factorT4 lysozymemethyl-beta-cyclodextrinmaltose-binding proteinOlfactory receptors
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Xuan Zheng, Shuangshuang Dong, Jie Zheng, Duanhua Li, Feng Li, Zhongli Luo,