Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231561 | Biotechnology Advances | 2013 | 14 Pages |
Abstract
In light of unrestricted use of first-generation penicillins, these antibiotics are now superseded by their semisynthetic counterparts for augmented antibiosis. Traditional penicillin chemistry involves the use of hazardous chemicals and harsh reaction conditions for the production of semisynthetic derivatives and, therefore, is being displaced by the biosynthetic platform using enzymatic transformations. Penicillin G acylase (PGA) is one of the most relevant and widely used biocatalysts for the industrial production of β-lactam semisynthetic antibiotics. Accordingly, considerable genetic and biochemical engineering strategies have been devoted towards PGA applications. This article provides a state-of-the-art review in recent biotechnological advances associated with PGA, particularly in the production technologies with an emphasis on using the Escherichia coli expression platform.
Keywords
tRNAHspSSPNTNSSCTCABRPIPTGRBSPAAmRNAPenicillin G7-ACAPENGPGAPenicillin V acylase6-APA7-ADCAGlutaryl acylasePVAd-amino acid oxidaseDNAPenicillin G acylaseamino acidβ-Lactam antibiotics7-Aminocephalosporanic aciddeoxyribonucleic acidtricarboxylic acidribonucleic acidRNAPhenylacetic acidEscherichia colidissolved oxygenisopropyl-β-d-thio-galactosideTATDAOribosome binding siteOuter membraneinner membranetrigger factorNucleotidesPosttranslational processingRecombinant proteinCRPheat-shock proteincAMP receptor proteinpolyethylene glycolPEGPenicillinPeptidoglycan
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Kajan Srirangan, Valerie Orr, Lamees Akawi, Adam Westbrook, Murray Moo-Young, C. Perry Chou,