Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231625 | Biotechnology Advances | 2014 | 12 Pages |
Abstract
A multi-stage continuous high cell density culture (MSC-HCDC) system makes it possible to achieve high productivity together with high product titer of many bioproducts. For long-term continuous operation of MSC-HCDC systems, the cell retention time and hydraulic retention time must be decoupled and strains (bacteria, yeast, plant, and animal cells) must be stable. MSC-HCDC systems are suitable for low-value high-volume extracellular products such as fuel ethanol, lactic acid or volatile fatty acids, and high-value products such as monoclonal antibodies as well as intracellular products such as polyhydroxybutyric acid (PHB), microbial lipids or a number of therapeutics. Better understanding of the fermentation kinetics of a specific product and reliable high-density culture methods for the product-generating microorganisms will facilitate timely industrialization of MSC-HCDC systems for products that are currently obtained in fed-batch bioreactors.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ho Nam Chang, Kwonsu Jung, Jin-dal-rae Choi, Joon Chul Lee, Hee-Chul Woo,