Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10231704 | Biotechnology Advances | 2005 | 15 Pages |
Abstract
Filamentous fungi have long been used for the production of metabolites and enzymes. With developments in genetic engineering and molecular biology, filamentous fungi have also achieved increased attention as hosts for recombinant DNA. However, the production levels of non-fungal proteins are usually low. Despite the achievements obtained using molecular tools, the heterologous protein loss caused by extracellular fungal protease degradation persists. This review provides an overview of the potential bioprocessing strategies that can be applied to inhibit protease activity thereby enhancing heterologous protein production.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Liping Wang, Darin Ridgway, Tingyue Gu, Murray Moo-Young,