Design-based re-engineering of biosynthetic gene clusters: plug-and-play in practice

- Microbial genomes encode thousands of biosynthetic gene clusters for unknown compounds.
- Such gene clusters can be computationally detected and prioritized effectively.
- Synthetic biology approaches allow comprehensive re-engineering of gene clusters.
- During re-engineering, native regulation can be replaced by synthetic regulation.
- Re-engineered gene clusters can be used for plug-and-play screening in optimized hosts.