| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10232422 | Current Opinion in Biotechnology | 2005 | 8 Pages |
Abstract
DNA and RNA polymerases have evolved in nature to function in specific environments with specific substrates. Thus, although the commercial availability of these enzymes has revolutionized the biotechnology industry, their applications are limited. The availability of polymerases that have unnatural properties would be of even greater utility. Towards this goal, several activity-based screening and selection approaches have been developed. Using these techniques, polymerases that synthesize a variety of different polymers, including those containing 2â²-O-methyl-modified nucleotides or unnatural base pairs, have been evolved. These results suggest that polymerases tailored for any specific application could soon be available.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Allison A Henry, Floyd E Romesberg,