Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10232483 | Current Opinion in Biotechnology | 2005 | 10 Pages |
Abstract
Improving the spatial resolution of optical microscopes is important for a vast number of applications in the life sciences. Optical microscopy allows intact samples and living cells to be studied in their natural environment, tasks that are not possible with other microscopy methods (e.g. electron microscopy). Major advances in the past two decades have significantly improved microscope resolution. By using interference and structured light methods microscope resolution has been improved to â¼100Â nm, and with non-linear methods a ten times improvement has been demonstrated to a current resolution limit of â¼30Â nm. These methods bring together old theoretical concepts such as interference with novel non-linear methods that improve spatial resolution beyond the limits that were previously assumed to be unreachable.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yuval Garini, Bart J Vermolen, Ian T Young,