Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10233170 | Enzyme and Microbial Technology | 2005 | 6 Pages |
Abstract
Diacetyl-chitobiose (GlcNAc)2 was produced exclusively using a Vibrio furnissii chitinase (chi E, 89Â kDa) hydrolysis of chitin treated with dimethylsulfoxide containing lithium chloride. This method represents a novel way for the preparation of colloidal chitin. Continuous diafiltration was used for the efficient recycling of enzyme and synthesis of (GlcNAc)2 on a preparative scale. Chitooligosaccharides were also synthesized in a transglycosylation reaction employing lysozyme from hen-egg white with diacetyl-chitobiose produced by a Vibrio furnissii chitinase (chi E, 89Â kDa) as a substrate in 50% 2-ethoxy ethyl ether co-solvent system. The co-solvent systems studied were triethyl phosphate, 2-ethoxy ethyl ether and acetone.
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Authors
Jung Hae Yoon,