| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10233191 | Enzyme and Microbial Technology | 2005 | 7 Pages |
Abstract
A psychrotolerant Bacillus sp. from Antarctica produced an alkaline phosphatase in the culture supernatant. The strain showed 98.4% 16s rDNA sequence identity with Bacillus sphaericus. The 76 kDa protein was purified 11.1-fold showing alkaline phosphomonoesterase activity. Enzyme was optimally produced at 25 °C and pH 7.0. This cold active alkaline phosphatase is heat labile and gets completely inactivated at 60 °C in 50 min and is active in broad pH range.
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Authors
Ram Kumar Dhaked, Syed Imteyaz Alam, Aparna Dixit, Lokendra Singh,