Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10233210 | Enzyme and Microbial Technology | 2005 | 7 Pages |
Abstract
An artificial bifunctional enzyme, xylanase-cellulase, has been prepared by gene fusion. Three chimeric genes were constructed that encoded fusion proteins of different lengths. The fusion proteins exhibited both xylanase (XynX) and cellulase (Cel5Z::Ω) activity when cel5Z::Ω was fused downstream of xynX, but not when xynX was fused downstream of cel5Z::Ω. Activities of bifunctional enzymes decreased when a shorter xylanase peptide was fused. Three fusion enzymes were purified, and the molecular weights of the enzymes were estimated by CMC-SDS-PAGE and XYN-SDS-PAGE to be 149, 129, and 87 kDa, respectively. The fusion enzymes displayed optimum cellulase activity at pH 8.0 and 50 °C and optimum xylanase activity at pH 8.0 and 70 °C.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Jin Mee An, Young Kyun Kim, Woo Jin Lim, Su Young Hong, Chang Long An, Eun Chule Shin, Kye Man Cho, Byoung Rock Choi, Jung Mi Kang, Sun Mi Lee, Hoon Kim, Han Dae Yun,