Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10233351 | Enzyme and Microbial Technology | 2005 | 4 Pages |
Abstract
In order to over express the xylA gene of Streptomyces sp. SK strain, it was cloned under the control of the constitutive ermE-up promoter. This construct was integrated through site-specific recombination process into the chromosome of a Streptomyces violaceoniger glucose isomerase deficient strain using the non-replicative vector pTS55. The resulting CBS4 strain shows a perfect stability in the absence of selection pressure. Its glucose isomerase activity was about four and nine-fold greater, than that obtained from Streptomyces sp. SK, respectively fully induced or not by xylose.
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Bioengineering
Authors
Monia Mezghani, Mohamed Ali Borgi, Radhouane Kammoun, Hedi Aouissaoui, Samir Bejar,