| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10234588 | Metabolic Engineering | 2012 | 8 Pages |
Abstract
⺠A mRNA secondary structure was designed to couple translation of two genes. ⺠System was successfully demonstrated with reporter genes and resistance markers. ⺠Translational coupling functions at high, medium, and low expression levels. ⺠Translational coupling generates a proportional translation response. ⺠The system was used to optimize conditions for expressing a â¼300 kDa protein.
Keywords
qPCRRFPRBSLICYFPACPPKSOLSEscherichia coliSDS-PAGEProtein expressionmRNA secondary structureribosome binding sitePolyketide synthaseSynthetic biologyChloramphenicol resistanceKanamycin resistancequantitative polymerase chain reactionacyl-carrier proteinyellow fluorescent proteinLigation independent cloningTranslational coupling
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Daniel Mendez-Perez, Suman Gunasekaran, Victor J. Orler, Brian F. Pfleger,