Article ID Journal Published Year Pages File Type
10234589 Metabolic Engineering 2012 11 Pages PDF
Abstract
► Synthetic RNA switches enable high throughput, in vivo screens for enzyme activity. ► Assays can be run in clonal culture by flow cytometry or in single cells by FACS. ► Seven rounds of screening by fluorescence improved enzyme activity in vivo 33 fold.
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Physical Sciences and Engineering Chemical Engineering Bioengineering
Preview
High-throughput enzyme evolution in Saccharomyces cerevisiae using a synthetic RNA switch
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