| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10234993 | New Biotechnology | 2015 | 8 Pages |
Abstract
- We used magnetic tweezers to apply forces to proteins in a sandwich immunoassay format.
- The force-induced dissociation rate was quantified in a cTn sandwich immunoassay.
- Interference of Pluronic F-127, a non-ionic surfactant, was studied.
- The surfactant increases the dissociation rate already at low concentrations.
- Circular dichroism data show that the surfactant influences the conformation of cTn.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
D. Dorokhin, L.J. van IJzendoorn, A.M. de Jong, L. Nieto, L. Brunsveld, J.G. Orsel, M.W.J. Prins,