Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235044 | New Biotechnology | 2014 | 10 Pages |
Abstract
Nuclease-based gene targeting (NBGT) represents a significant breakthrough in targeted genome editing since it is applicable from single-celled protozoa to human, including several species of economic importance. Along with the fast progress in NBGT and the increasing availability of customized nucleases, more data are available about off-target effects associated with the use of this approach. We discuss how NBGT may offer a new perspective for genetic modification, we address some aspects crucial for a safety improvement of the corresponding techniques and we also briefly relate the use of NBGT applications and products to the regulatory oversight.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Katia Pauwels, Nancy Podevin, Didier Breyer, Dana Carroll, Philippe Herman,