| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235082 | New Biotechnology | 2012 | 8 Pages |
Abstract
⺠We review studies on antibody array analysis of labelled proteomes. ⺠We point to the limitations of assays based on detection with a single binder and a protein label and argue that direct evidence for specificity is lacking in the literature. ⺠We present a new variant of the assay referred to as Size-MAP (Size-exclusion chromatography-resolved Microsphere-based Affinity Proteomics). ⺠Size-MAP resembles a multiplexed western blot and provides internal reference of specificity in highly multiplexed protein analysis.
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Authors
Anders Holm, Weiwei Wu, Fridtjof Lund-Johansen,