Modulation of transglycosylation and improved malto-oligosaccharide synthesis by protein engineering of maltogenic amylase from Bacillus lehensis G1

- Rational design using a homology-modeled structure of MAG1 was performed.
- Mutation caused active site modification to accommodate longer donor/acceptor.
- Increased active site hydrophobicity caused reduction in maltotriose concentration needed for transglycosylation.
- A malto-oligosaccharide with a higher degree of polymerization was synthesized.