| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235194 | Process Biochemistry | 2015 | 5 Pages |
Abstract
- NanoLuc was developed as a novel fusion partner for soluble overexpression in E. coli.
- The aggregation-prone IL6 was used as an example for difficult-to-express proteins.
- Soluble 6ÃHis-NanoLuc-IL6 was overexpressed in E. coli and purified to homogeneity.
- Fully active IL6 was obtained at a high yield after removal of the fusion partner.
- The NanoLuc-fusion approach could be applied to other aggregation-prone proteins.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ben-Jun Ji, Ge Song, Zhou Zhang, Zhan-Yun Guo,