Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235209 | Process Biochemistry | 2015 | 40 Pages |
Abstract
- We establish a system for expressing Tuna AI as tandem multimer in Escherichia coli.
- The fusion protein Fusion-32TunaAI shows the highest expression level.
- The obtained Tuna AI is confirmed and shows high activity in vitro and in vivo.
- We establish two economic protocols for protein and peptide purification.
Keywords
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yunliang Li, Bei Wang, Henan Zhang, Zhigao Wang, Shanying Zhu, Haile Ma,