| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10235242 | Process Biochemistry | 2015 | 8 Pages |
Abstract
- Analysis of protein surface property was used for rational selection of downstream steps of proteins.
- Downstream steps for purification of rHLF from milk of transgenic cow were selected.
- Casein was removed by calcium precipitation leaving rHLF in the supernatant.
- One step cationic ion exchange chromatography realized complete separation of rHLF from BLF.
- The purification process was scaled up to pilot-plant scale of 500Â l milk of transgenic cow per batch.
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Yan Zhang, Jian Luo, Songping Zhang, Qian Bai, Zenglan Li, Qiang Li, Yongdong Liu, Guifeng Zhang, Guanghui Ma, Zhiguo Su,