Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235244 | Process Biochemistry | 2015 | 43 Pages |
Abstract
- Novel ATPS composed of THF and K2HPO4/KH2PO4 at pH 7 was studied.
- The phase diagram, tie-lines and critical point were determined at 298Â K.
- The concentration of components and temperature of equilibrium was investigated.
- The enzyme partitioning is spontaneous and governed by entropic effects.
- Lipase from Bacillus produced by submerged fermentation was purified 103.9 (fold).
Related Topics
Physical Sciences and Engineering
Chemical Engineering
Bioengineering
Authors
Ranyere L. Souza, Rafaella A. Lima, João A.P. Coutinho, Cleide M.F. Soares, Álvaro S. Lima,