Purification and characterization of a novel antioxidant peptide from bovine hair hydrolysates

Article ID	Journal	Published Year	Pages	File Type
10235257	Process Biochemistry	2015	35 Pages	PDF

Abstract

- A novel antioxidant peptide (BHP) was isolated from bovine hair hydrolysates.
- BHP exhibited the remarkable antioxidant activity to scavenge free radicals.
- BHP could inhibit the erythrocyte hemolysis and lipid peroxidation effectively.
- BHP possessed the ability to inhibit the oxidative damage of DNA and PC12 cells.

Keywords

ABTS Cys GSH GFC BHA HPSEC THR TBHQ Bovine hair PBS 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide DMSO DNA MTT ROS Arginine Oxidative injury Arg glutamic acid SER bhp threonine Characterization HIS Dimethyl sulfoxide Serine Cysteine phosphate buffer saline Antioxidant activity matrix assisted laser desorption ionization time-of-flight mass spectrometry MALDI-TOF-MS PRO butylated hydroxyanisole histidine Purification Proline Peptide gel filtration chromatography high performance size exclusion chromatography Glu Glutathione Reactive oxygen species