Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10235305 | Process Biochemistry | 2014 | 4 Pages |
Abstract
The oxygenic photosynthetic prokaryotes cyanobacteria have recently attracted worldwide interest in production of biofuels and bioactive natural compounds. Disruption of cells is a prerequisite for extraction of intracellular compounds. However, cyanobacterial cells are difficult to be disrupted because of the multiple-layered cell walls covered with a mucilaginous sheath. Here, we report a new disruption method for cyanobacteria, where an abrasive material, silicon carbide, is ground with cell pellet in situ. A cell disruption efficiency of 93.3 ± 2.3% was achieved in 6 min, an efficiency comparable to that obtained after 30 min of sonication. The new method yielded crude cell extracts with high concentrations of protein and high activity of the target enzyme, d-lactate dehydrogenase. This method has potential to be used for disruption of cells of photosynthetic microorganisms on a larger scale.
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Authors
Jie Zhou, Fuliang Zhang, Hengkai Meng, Guanhui Bao, Yanping Zhang, Yin Li,